Bohdan OSTASH, PhD
Senior researcher, laboratory of genetics, selection and genetic engineering of antibiotic producers (NDL-42);
Professor (part-time), Department of Genetics and Biotechnology
Born and raised in Ukraine, at the foot of Carpathian Mountains, as per cultural norm I entered Ivan Franko National University of Lviv (IFNUL). I got my undergraduate degree in Genetics from IFNUL in 1999. I spent the next four years working towards a Ph.D. on understanding the biosynthesis of antitumor antibiotic landomycin E. I did this by making and analyzing knockouts of individual genes for landomycin biosynthesis in actinobacterium Streptomyces globisporus 1912. Since 2003 I have worked in the field of actinobacterial genetics and genomics, first as a postdoctoral fellow in the lab of Suzanne Walker (Harvard Medical School), and then as an independent researcher at IFNUL. In 2011 I took part-time teaching position at the Department of Genetics and Biotechnology of IFNUL. I teach courses in genomics and bionformatics.
My research and that of my PhD students is mainly focused on genetics and evolution of microbial (particularly actinobacterial) secondary metabolism. A practical outcome of these investigations is the development of genetic approaches towards in vivo manipulations of structures of small molecules being studied and improvement of their production in natural and heterologous hosts. As the secondary metabolism depends on primary metabolites flux, certain aspects of primary metabolism are also investigated. Particularly, we are currently interested in the genetics of tRNA posttranscriptional modifications and sulfur cycle in actinobacteria, which remain terra incognita on metabolic maps of Actinobacteria. Lastly, we’re trying to understand the origins of unequal usage of synonymous codons in microbial genomes, with a focus on actinobacterial ones. Our primary approach is genetic; once the hypotheses take shape the other techniques are employed. Full list of publications and access options can be viewed at www.ncbi.nlm.nih.gov/pubmed?term=ostash%20b; www.researchgate.net/profile/Bohdan_Ostash.
Selected publications (2013-2015)
- Tsypik O, Yushchuk O, Zaburannyi N, Flardh K, Walker S, Fedorenko V, Ostash B. Transcriptional regulators of GntR family in Streptomyces coelicolor A3(2): analysis in silico and in vivo of YtrA subfamily. Folia Microbiol. 2015 Oct 3. [Epub ahead of print]. doi: 10.1007/s12223-015-0426-7. PubMed PMID: 26433722.
- Medema MH, Kottmann R, Yilmaz P, [115 authors], Ostash B, et al. Minimum information about a biosynthetic gene cluster. Nat Chem Biol. 2015 Aug 18;11(9):625-31. doi: 10.1038/nchembio.1890. PubMed PMID: 26284661.
- Ostash B, Yushchuk O, Tistechok S, Mutenko H, Horbal L, Muryn A, Dacyuk Y, Kalinowski J, Luzhetskyy A, Fedorenko V. The adpA-like regulatory gene from Actinoplanes teichomyceticus: in silico analysis and heterologous expression. World J Microbiol Biotechnol. 2015 Aug;31(8):1297-301. doi: 10.1007/s11274-015-1882-6. Epub 2015 Jun 4. PubMed PMID: 26041369.
- Horbal L, Kobylyanskyy A, Truman AW, Zaburranyi N, Ostash B, Luzhetskyy A, Marinelli F, Fedorenko V. The pathway-specific regulatory genes, tei15* and tei16*, are the master switches of teicoplanin production in Actinoplanes teichomyceticus. Appl Microbiol Biotechnol. 2014 Nov;98(22):9295-309. doi: 10.1007/s00253-014-5969-z. Epub 2014 Aug 9. PubMed PMID: 25104028.
- Zaburannyi N, Rabyk M, Ostash B, Fedorenko V, Luzhetskyy A. Insights into naturally minimised Streptomyces albus J1074 genome. BMC Genomics. 2014 Feb 5;15:97. doi: 10.1186/1471-2164-15-97. PubMed PMID: 24495463; PubMed Central PMCID: PMC3937824.
- Ostash B, Campbell J, Luzhetskyy A, Walker S. MoeH5: a natural glycorandomizer from the moenomycin biosynthetic pathway. Mol Microbiol. 2013 Dec;90(6):1324-38. doi: 10.1111/mmi.12437. Epub 2013 Nov 21. PubMed PMID: 24164498; PubMed Central PMCID: PMC3961715.
- Horbal L, Kobylyanskyy A, Yushchuk O, Zaburannyi N, Luzhetskyy A, Ostash B, Marinelli F, Fedorenko V. Evaluation of heterologous promoters for genetic analysis of Actinoplanes teichomyceticus – producer of teicoplanin, drug of last defense. J Biotechnol. 2013 Dec;168(4):367-72. doi: 10.1016/j.jbiotec.2013.10.018. Epub 2013 Oct 24. PubMed PMID: 24161919.
- Makitrynskyy R, Ostash B, Tsypik O, Rebets Y, Doud E, Meredith T, Luzhetskyy A, Bechthold A, Walker S, Fedorenko V. Pleiotropic regulatory genes bldA, adpA and absB are implicated in production of phosphoglycolipid antibiotic moenomycin. Open Biol. 2013 Oct 23;3(10):130121. doi: 10.1098/rsob.130121. PubMed PMID: 24153004; PubMed Central PMCID: PMC3814723.
- Ostash B, Gren T, Hrubskyy Y, Tistechok S, Beshley S, Baranov V, Fedorenko V. Cultivable actinomycetes from rhizosphere of birch (Betula pendula) growing on a coal mine dump in Silets, Ukraine. J Basic Microbiol. 2014 Aug;54(8):851-7. doi: 10.1002/jobm.201200551. Epub 2013 May 17. PubMed PMID: 23686352.