Viсtor Fedorenko

Head of the Department, Doctor of Biological Sciences, Professor Phone: +38-032-2394768 +38-032-2394768 Fax: +38-032-2394475 E-mail: v_fedorenko@franko.lviv.ua

Born in 1951.
1973: has graduated from Ivan Franko Lviv University, Ukraine
1976 - 1979: Post-graduate course in All-Union Scientific-Research Institute of Genetics and Selection of Industrial Microorganisms (Moscow, Russia) under the supervision of Professor N.D. Lomovskaya
1979 - 1984: Assistant lecturer at the Department of Genetics and Biotechnology of Ivan Franko Lviv National University
1984 - 1994: Associate professor at the same Department
1987 - present Project Leader, Department of Genetics and Biotechnology of Ivan Franko Lviv National University
1987-1991- Research Fellow at the National Scientific Centre of Antibiotics (Moscow, Russia)
1995-1997 - Research Fellow at the Hans-Knoell Institute for Natural Product Research (Jena, Germany)
1994 - present: Head of the Department of Genetics and Biotechnology of Ivan Franko Lviv National University
1995 – present: Scientific supervisor of Microbial Culture Collection of antibiotic producer, Ivan Franko Lviv National University
Current courses

• Genetics
• Microorganisms genetics
• Genetic Engineering
• Methods of molecular cloning

Research Interests
Genetics, selection and genetic engineering of actinomycetes, genetic control of antibiotic biosynthesis and resistance, mechanisms of bacterial genome instability.
Group Members
Dr. Bohdan Ostash
Dr. Oleksandr Gromyko
Dr. Iryna Ostash
Dr. Lilia Gorbal
Oleksandra Aravitska
Jaroslav Grubskyy
Roman Makitrynskyy
Mariia Rabyk
Nestor Zaburannyi
Olga Tsypik
Tetyana Gren
Maria Lopatniuk
Halina Mutenko
Major publication list for 2004-2011

• Ostash B., Rebets Y., Myronovskyy M., Tsypik O., Ostash I., Kulachkovskyy O., Datsyuk Y., Nakamura T., Walker S., Fedorenko V. Identification and characterization of the Streptomyces globisporus 1912 regulatory gene lndYR that affects sporulation and antibiotic production. Microbiology. – 2011. – Vol. 157. –  P. 1240 – 1249.
•  Myronovskyi M. Welle E., Fedorenko V., Luzhetskyy A. Beta-glucuronidase as a sensitive and versatile reporter in actinomycetes.Appl. Environ. Microbiol. – 2011. – Vol. 77, Is. 15. – P. 5420–5427.
• Rabyk M. B. Ostash, Y. Rebets, S. Walker, V. Fedorenko. Streptomyces ghanaensis pleiotropic regulatory gene wblAgh influences morphogenesis and moenomycin production. Biotechnol. Lett. – 2011. – Vol. 33. – P. 2481 – 2486.

4. Klimishin D.A. Rabyk M.V., Gren’ T.P., Nimets’ O.Ya., Gonchar M.A., Gromyko A.N.,   Fedorenko V.A. Design of StreptomycesnogalaterLV65 strains with higher synthesis of nogalamicin using regulatory genes.  Appl. Biochem. Microbiol. (Moscow) – 2011. – Vol. 47, No. 6. – Р.  594–598.
5. Klymyshin D.O., Gren T.P., Fedorenko V.O. Role of the snorA gene in nogalamycin biosynthesis by strain Streptomyces nogalater Lv65. Microbiology (Moscow) -  2011. - Vol. 80, No. 4 -  P. 496–501.
6. Ostash B., Yan X., Fedorenko V., Bechthold A. Chemoenzymatic and bioenzymatic synthesis of carbohydrate containing natural products. Top. Curr. Chem. "Natural products via enzymatic reactions". Ed. J. Piel. – Springer-Verlag Berlin Heidelberg : 2010. - Vol. 297. - P. 105–148.
7. Ostash B., Doud E., Fedorenko V. The molecular biology of moenomycins: towards novel antibiotics based on inhibition of bacterial peptidoglycan glycosyltransferases. Biol. Chem. 2010. – Vol. 391, No. 5. – P.4 99–504.
8. Ostash B., Ostash I., Zhu L., Kharel M.K., Luzhetskyy A., Behthold A., Walker S., Rohr J., Fedorenko V. Properties of lank-based regulatory circuit involved in landomycin biosynthesis in Streptomyces cyanogenus. Rus. J. Genet. – 2010. – Vol. 46, No. 5. – P. 530–535. [pdf, 347Kb]
9. Myronovskyy M.L., Ostash B.E., Fedorenko V.A. Diversity of genes encoding nonribosomal peptide synthetases in the Streptomyces sioyaensis genome. Rus. J. Genet. – 2010. – Vol. 46, No.7. – P.791–797.
10. Makitrynskyy R., Rebets Y., Ostash B., Zaburannyi N., Rabyk M., Walker S., Fedorenko V. Genetic factors that influence moenomycin production in streptomycetes. J. Ind. Microbiol. Biotechnol. – 2010. – Vol. 37, No. 6. – P. 599–566.
11. Horbal L., Rebets Y., Rabyk M., Luzhetskyy A., Welle E., Ostash B., NakamuraT., Fedorenko V., Bechthold A. Characterization and analysis of the regulatory network involved in control of lipomycin biosynthesis in Streptomyces aureofaciens Tu117. Appl. Microbiol. Biotechnol. – 2010. – Vol. 85, No. 4. – P. 1069–1079.
12. Zaburannyy N., Ostash B., Fedorenko V. TTA Lynx: a web-based service for analysis of actinomycete genes containing rare TTA codon. Bioinformatics – 2009. – Vol. 25, No.18 – P. 2432–2433.
13. Ostash B., Makitrinskyy R., Walker S., Fedorenko V. Identification and characterization of Streptomyces ghanaensis ATCC14672 integration sites for three actinophage-based plasmids. Plasmid – 2009. – Vol. 61, No.3. – P.171–175.
14. Myronovskyy M., Ostash B., Ostash I., Fedorenko V. A gene cloning system for the siomycin producer Streptomyces sioyaensis NRRL-B5408.. Folia Microbiologica – 2009. – Vol. 54, No. 2 – Р. 91–96.
15. Kobylyanskyy A., Ostash B., Fedorenko V. Heterologous cross-expression of oxygenase and glycosyltransferase genes in streptomycetes, producing angucyclic antibiotics. Cytology and Genetics – 2009. – Vol. 43, No. 3. – P. 194–200.
16. Ostash B., Korynevska A., Stoika R., Fedorenko V. Chemistry and biology of landomycins, an expanding family of polyketide natural products. Mini-Rev. Med. Chem. – 2009. – Vol. 9, No. 9. – P. 1040–1051.
17. Rebets Y., Horbal L., Boll R., Fedorenko V., Bechthold A. Production of аvilamycin A is regulated by AviC1 and AviC2, two transcriptional activators. J. Antibiot. – 2009. – Vol. 62, No. 8 - P. 461–464.
18. Rebets Y., Dutko L., Ostash B., Luzhetskyy A., Kulachkovskyy O., Yamaguchi T., Nakamura T., Bechthold A., Fedorenko V. Function of lanI in regulation of landomycin A biosynthesis in Streptomyces cyanogenus S136 and cross-complementation studies with Streptomyces antibiotic regulatory proteins encoding genes. Arch. Microbiol. – 2008. – Vol. 190, No. 1. – P. 105–109. [pdf, 455Kb]
19. Ostash I., Rebets Y., Ostash B., Kobylyanskyy A., Myronovskyy M., Nakamura T., Walker S., Fedorenko V. An ABC transporter encoding gene lndW confers resistance to landomycin E. Arch Microbiol. – 2008. – Vol. 190, No. 1. – P. 105– 109. [pdf, 291Kb]
20. Ostash I., Ostash B., Luzhetskyy A., Bechthold A., Walker S., Fedorenko V. Coordination of export and glycosilation of landomycins in Streptomyces cyanogenus S136. FEMS Microbiol. Lett. – 2008. – Vol. 285, No 2. – P. 195–202. [pdf, 284Kb]
21. Fedorenko V., Chernyk Ia., Маksymiv D., Bodnar L. Genetics problems and exercises. – Lviv: Оriana Nova, 2008. – 598 p. Ukrainian.
22. Ostash I., Ostash B., Walker S., Fedorenko V. Proton-dependent transporter gene, lndJ, confers resistance to landomycin E in Streptomyces globisporus. Rus. J. Genet. –2007. – Vol. 43, No. 8. – P. 852–857.
23. Fedorenko V., Ostash B., Gonchar M., Rebets Y. A practical course of genetics, genetic engineering and analytical biotechnology of microorganisms. - Lviv: Ivan Franko National University Publishing Group, 2007. – 279 p. Ukrainian.
24. Klymyshyn D.O., Gromyko O.V., Fedorenko V.O. Application of intergeneric conjugation of Esherichia coli - Streptomyces for transfer of recombinant DNA into the strain S. nogalater IMET43360. Cytology and Genetics – 2007. – Vol. 41, N5. – P. 263–267. Ukrainian.
25. Dutko L., Rebets Y., Ostash B., Luzhetskyy A., Bechthold A., Nakamura T., Fedorenko V. A putative proteinase gene is involved in regulation of landomycin E biosynthesis in Streptomyces globisporus 1912. FEMS Microbiol. Letters – 2006. – Vol. 255, Is.2. – P. 280 - 285 [pdf, 149Kb].
26. Rebets Y., Ostash B., Fukachara M., Nakamura T., Fedorenko V. Expression of the regulatory protein LndI for landomycin E production in Streptomyces globisporus 1912 is controlled by the availability of tRNA for the the rare UUA codon. FEMS Microbiol. Letters – 2006. –Vol. 256, Is.1. – P. 30–37 [pdf, 451Kb].
27. Luzhetskyy A., Fedoryshyn M., Gromyko O., Ostash B., Rebets Y., Bechthold A., Fedorenko V. IncP plasmids are most effective in mediating conjugation between Escherichia coli and Streptomycetes. Rus. J. Genet. – 2006. – Vol. 42, No.5. – P. 476–481.
28. Baig I., Kharel M., Kobylyanskyy A., Zhu L., Rebets Y., Ostash B., Luzhetskyy A., Bechthold A., Fedorenko V., Rohr J. On the acceptor substrate of C-glycosyltransferase UrdGT2: three prejadomycin C-glycosides from an engineered mutant of Streptomyces globisporus 1912 delta lndE(urdGT2). Angew. Chem. Int. Ed. – 2006. – Vol. 45. – P. 7842–7846 [pdf, 240Kb].
29. Rebets Yu., Ostash B., Luzhetskyy A., Kushnir S. , Fukuhara M., Bechthold A., Nashimoto M., Nakamura T., Fedorenko V. DNA-binding activity of LndI protein and temporal expression of the gene that upregulates landomycin E production in Streptomyces globisporus 1912. Microbiology. – 2005. – Vol. 151, No 1. – P. 281–290 [pdf, 876Kb].
30. Zhu L., Ostash B., Rix U., Nur-E-Alam M., Mayers A., Luzhetskyy A., Mendez C., Salas J.A., Bechthold A., Fedorenko V., Rohr J. Identification of the function of gene lndM2 encoding a bifunctional oxygenase-reductase involved in the biosynthesis of the antitumor antibiotic landomycin E by Streptomyces globisporus 1912 supports the originally assigned structure for landomycinone. J.Org.Chem. – 2005. – Vol. 70, No 2. – P. 631–638 [pdf, 209Kb].
31. Luzhetskyy A., Zhu L., Gibson M., Fedoryshyn M., Durr C., Hofman C., Hoffmeister D., Ostash B., Mattingly C., Adams V., Fedorenko V., Rohr J., Bechthold A. Generation of novel landomycins M and O through targeted gene disruption. ChemBioChem.-2005.-Vol.6,N4.-P.675-678 [pdf, 87Kb].
32. Ostash B., Kozarevska I., Fedorenko V. Generation of landomycin D-producing strain Streptomyces globisporus LD3 // Folia Microbiol.-2005.-Vol.50,N1.-P.19-23 [pdf, 217Kb].
33. Ostash B.E., Ogonian S.V., Luzhetskyy A.N., Bechthold A., Fedorenko V.A. The use of PCR for detecting genes that encode type I polyketide synthases in genomes of Actinomycetes. Rus J Genet. -2005.- Vol. 41, N5.- P.473-478.
34. Ostash B., Rix U., Remsing Rix L.L., Tao Liu, Lombo F., Luzhetskyy A., Gromyko O., Wang C., Brana A.F., Mendez C., Salas J.A., Fedorenko V., Rohr J. Generation of novel landomycins by combinatorial biosynthetic manipulation of the lndGT4 gene of the landopmycin E cluster in S. globisporus. Chemistry and Biology-2004.-Vol.11.-N.4.-P.547-555 [pdf, 645Kb].
35. Gromyko O., Rebets Y., Ostash B., Luzhetskyy A., Fukuchara M., Bechtold A., Nakamura T., Fedorenko V. Generation of Streptomyces globisporus SMY622 strain with increased landomycin E production and it's initial characterization. J.Antibiot.-2004.-Vol.57.-N.6-P.383-389 [pdf, 292Kb].
36. Luzhetskyy A., Lin T., Fedoryshyn M., Ostash B., Fedorenko V., Rohr J., Bechthold A. Function of lanGT3 a glycosyltransferase gene involved in landomycin A biosynthesis. Chembiochem.-2004.-Vol.5, N11.-P.1567- 1570 [pdf, 131Kb].